The study of drug-human serum albumin binding affinity is considered as an important topic in the drug discovery and development. Capillary electrophoresis based on the principles of frontal analysis (CE-FA) is one of the most important technique, due to the short analysis time, high separation efficiency, low sample requirement, easy computerization, robustness, precision of results, low cost sample throughput and versatility to study multiple equilibria. This method is used to study the...
Dr. Warren Tay (1843-1927) and Dr. Bernard Sachs (1858-1944) were the two men for which Tay-Sachs disease was named. (U.S. National Library of Medicine 2010). In 1881, Dr. Warren Tay was an ophthalmologist from Britain who first identified a patient with a cherry-red spot on the retina of their eye (Mayo Clinic 2010). A neurologist from New York, Bernard Sachs, worked a few years later and gave the first report on the cellular basis of Tay-Sachs disease. Sachs also realized that the...
BIO450 Primer Design Tutorial The most critical step in your PCR experiment will be designing your oligonucleotide primers. Poor primers could result in little or even no PCR product. Alternatively, they could amplify many unwanted DNA fragments. Either way, it would interfere in subsequence cloning steps. Therefore it is critical that you design your primers carefully. Primer design requires extensive computer-based sequence analysis and this tutorial is designed to lead you through that...
This experiment was designated to investigate the effect of temperature on metabolic rate of lucilia sericata larvae. Water bath has been used to set up the different temperatures of water at 300C, 350C, 400C, 450C, 500C and 550C. lucilia sericata larvae were chosen to show the effect of different temperature on biological reaction of organism. The active lucilia sericata larvae of the same size were soaked in different temperature of water for about 30 seconds and the number of wriggles was...
Many distinctive properties of Green Fluorescent Protein (GFP) such as its ability to generate an internal fluorophore make it extra-ordinary. This review will set out to discuss not only the structural and biochemical properties, uses and interesting features of this unique protein but also the applications of new variants of GFP that have been developed. INTRODUCTION GFP comprises of 238 amino acids, absorbs blue light (Amax 395 nm) to fluoresce green light (Imax 509 nm) (Miller et al, 2008)....
Cotton is the most important cash crop of Pakistan known as "white gold" (Ahmad, 2007). Pakistan is the fourth largest producer of cotton in the world, third largest exporter of raw cotton, fourth largest consumer of cotton, and the largest exporter of cotton yarn. 1.3 million farmers (out of a total of 5 million) cultivate cotton over 3 million hectares, covering 15 per cent of the cultivable area in the country (Abro et al., 2004). Economy of Pakistan is heavily dependent on the...
Counter-current chromatography (CCC) is a separation technique applying liquid-liquid phase. It is carried out without the aid of a solid supporting matrix to retain the stationary liquid in the chromatographic column (Conway, 1991). Separation of analytes in the sample is based on the affinities of mobile and stationary phases of the chromatography mode. CCC is carrying out based on three different stages. First are mixing, then settling; and lastly separation of analytes in the sample...
Mycobacteria are acid fast bacilli which is due to the high lipid content of their cell wall.They are neither gram positive or gram negative. Mycobacteria is a genus within the order Actinomycetales that comprise a large number of well characterised species. The major pathogens are Mycobacterium Tuberculosis the cause of tuberculosis and mycobacterium leprae, the cause of leprosy .Atypical mycobacteria such as Mycobacterium avium-intracellular complex and Mycobacterium kansaii, can cause...
The initial description and measurement of Nuclear Magnetic Resonance was done by Isidor Rabi in the year 1938, for which he was honored with Nobel Prize in Physics for his outstanding contribution. In 1946, two physicists, namely, Felix Bloch and Edward Mills Purcell went further to use this on liquids and solids. They were feted with Nobel Prize in Physics for their stellar work in 1952. Isidor Rabi made the observation that a nucleus is said to be being in resonance when RF energy bearing a...
Aspirin, Paracetamol and Ibuprofen are examples of commonly used NSAIDS (non-steroidal anti-inflammatory drugs). They are the most widely used therapeutic drugs used by the general public today mainly for muscle complaints and relief of pain. NSAIDS vary in there action, strength, potency and the way in which they react in the body and are eliminated.[ [] ] An NSAID such as Aspirin, largely an analgesic has many pharmacological effects in the body which are anti-inflammatory,...