Diarrhoeal disease accounts for an estimated 2 billion episodes of illness and 1.5 million infant deaths globally each year (1). Such an evident impact of this illness on human mortality and morbidity requires the determination of optimal methods for overall management of enteropathogenic illness worldwide. Classed as the passing of three or more abnormally liquid or unformed stools per day (2a), diarrhoea is most commonly a symptom of gastrointestinal infection and the pathogens involved are...
In this study, Cd-induced apoptosis in human HEK-293T human embryonic kidney 293 cells was tested Surface membrane alteration was measured as a characteristic feature of apoptotic cells. For this purpose two staining were employed at the same time, Annexin V-FITC, and propidium iodide (PI). The concentration of Cd (5 μM) was found to induce apotosis in HEK-293T cell lines, using dose dependent method after 24 hour treatment. The percentage of apoptotic cells were 3.36, 5.5, 34.63 for...
The SUNFLOWER seeds have diuretic and expectorant properties and have been employed with success in the treatment of bronchial, laryngeal and pulmonary affections, coughs and colds, also in whooping cough. The oil possesses similar properties and may be given in doses of 10 to 15 drops or more, two or three times a day for coughs and colds, also in whooping cough. . A tincture of the leaves has been recommended in combination with balsamics in the treatment of bronchiectasis. The seeds, if...
Twelve extracts of Ludwigia octovalvis were examined to determine their total phenolic content (TPC), as well as antioxidant and antibacterial activities. The highest TPC and antioxidant activities (evaluated by 2, 2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assays) were detected in 80% methanol extract of the leaf; these are 264.76 ± 0.23 GAE mg/g d.w., 1080.84±6.07 µM TE/mg d.w., and 1256.88±5.38 µM TE/mg d.w. A strong correlation between...
Abstract- Methods for the diagnosis of infectious diseases have crawled in the last 2-3 decades. Many tests that form the backbone of the "modern" microbiology laboratory are based on very old and labour-intensive technologies such as microscopy. Innovation and implementation of PCR had lead to major advances in area of research and diagnostics. However, it requires a thermocycler and longer time to separate two DNA strands and amplify the required fragment. Burning need of the hour...
The aim of this project is to investigate the reaction catalysed in some amino acids. In which the reversibility of the enzyme was to be determined. And to determine wether the reaction goes to equilibrium in the forward and reverse reaction.IntroductionKey facts: Transaminases catalyse reversible reactions which inter-convert an α-amino acid and an α-keto acid into corresponding α-keto acid and α-amino acid respectively. In transamination the α-amino acid group of an α-amino acid is...
Aging is a developmental process that begins at conception and ends at death. Since everyone ages at different rate with body systems and organs aging at different rates in different individuals, it is impossible to categorize people based on chronological age alone. Aging is becoming older; the process that is genetically determined and environmentally modulated. As we age, our body changes in many ways that affect the function of both individual cells and organ systems. These changes,...
Solvents like ethanol, petroleum ether, ethyl acetate, butanol, methanol, toluene, formic acid, glacial acetic acid, acetonitrile, dimethyl sulphoxide and Folin- Ciocalteu's were purchased from SD fine chem. Ltd, Mumbai. Quercetin, Gallic acid, Rutin, 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and ascorbic acid (vitamin C) Dulbecco's modified eagle's medium (DMEM), Eagles minimum essential medium (EMEM), Trypsin, EDTA, Sulphorhodamine blue, Trichloro acetic acid, Fetal calf serum and...
Any change in copy number involves change in chromosome structure which eventually ends up joining two formerly separated DNA sequences, and is often mediated by DNA rearrangements. Genomic rearrangements involving gain, loss or disruption of dosage sensitive gene influence the phenotype of an organism through duplication, deletion, position effects and gene fusion or conversion events and may lead to genomic disorders [1, 3]. Since DNA rearrangements define DNA changes involving thousand to...
Pauling and Corey were able to develop the precise structure of the peptide bond from the x-ray diffraction patterns of keratins. They found out that the length of the carbon nitrogen bond of proteins was shorter than the actual carbon nitrogen bond. They were also able to describe the presence of four atoms in the peptide bond and pointed out that the two α carbon atoms lie in the same plane such that the oxygen atom of the carbonyl carbon and that the hydrogen atom of the nitrogen lie...