Since the introduction of GM crops in 1995, the debate of using such technology never cease and the acceptance of GM foods varies among region. Until very recently both supporting and anti-biotech groups were using propaganda style technique to gain grounds in public opinion, by this approach both parties induce more confusion rather than giving useful information to the public. Furthermore, the debate surrounding biotechnology and GMO related issues often focus on normative arguments rather...
CBCT is a relatively recent technology. It is a medical image acquisition technique based on a divergent pyramidal- or cone-shaped source of ionizing radiation directed through the middle of the area of interest onto a two-dimensional detector area on the opposite side. Among the first clinical application were single photon emission computerized tomography (SPECT) and angiography but more recent medical applications have included image guided radiotherapy and mammography. Imaging is...
Arsenic is a metalloid that occurs naturally in the earth and in the seas. It is odorless and tasteless. Arsenic is not an abundant element in the earth's crust, the average crustal concentration of 1.8 mg/kg ranks it fifty-two between tin and molybdenum (Demayo, 1985). However, through geogenic processing of crustal materials, arsenic can be concentrated in soils to a typical range of 2 to 20 mg/kg (Yan-Chu, 1994). In nature, it is widely distributed in a number of minerals, mainly as the...
Green technology recently drags major attention that makes the development of natural and renewable based products are on the rise. Native biopolymers matrices such as starch, natural resin, chitosan, bacterial cellulose and others are generally have low perfomance in term of strength, oxygen barriers and water absorption. Many attempts were conducted to enhance the performance of biopolymers. Focusing on green material development, reinforcement material from natural fiber was studied. ...
Kinetics of HLADM and HLA-DR association in wild-type Pala cells. Cells were pulse labeled with methionine for 15 min and chased in the presence of an excess of methionine and cysteine for the indicated periods of time (h). Class II and DM molecules were immunoprecipitated at each time point from CHAPS or Triton X-100 solubilized lysates (as indicated above each gel) with the DR-specific mAb L243 or with a polyclonal rabbit antiserum sp ecific for the DM heterodimer (αDM) and analyzed by...
In 1980, the United States Supreme Court heard a case titled Diamond, Commissioner of Patents and Trademarks v. Chakrabarty (United..."Diamond" par. 1). The issue in question was over a patent application filed by A.M. Chakrabarty, a genetic biologist working for General Electric (GE). Chakrabarty was working on a project that proposed to use a family of bacteria called Pseudomonas to clean up oil spills. Chakrabarty had engineered a new type of pseudomonad by combining plasmids...
Abstract: The inception of the concept for electrophoresis was made in 1807. After which it has been a staple tool used by biologist and chemist over the centuries. Various systems of electrophoresis have been introduced, from paper electrophoresis to today's modern automated electrophoresis system. The development of electrophoresis systems have been driven by the advancement of technology and also by the requirement for better and faster resolution of results. This paper reviews the...
Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), also known as Human herpes virus 1 and 2 (HHV-1 and -2), are two members of the herpes virus family, Herpesviridae, that infect humans. Both HSV-1 and -2 are ubiquitous and contagious (Shors, 409). They can be spread when an infected person is producing and shedding the virus. This virus has been interest of all scientists for many years and many studies have been done. The article titled "Simian TRIM5α proteins reduce replication of herpes...
Recombinant DNA technology is used for cloning of the genes of interest. The cloning involves isolation, restriction, ligation and insertion of recombinant DNA molecule into a host such as E. coli, yeast, an animal or a plant cell. In the following experiment phage λ and pUC19 were joined together by using DNA ligase followed by restriction digestion by EcoRI. The restriction and ligation products were analysed by agarose gel electrophoresis. The products of ligation were used to insert...
Smooth muscle cell (SMC) contraction is regulated mainly by receptor and mechanical activation of the contractile proteins actin and myosin. Contraction can also be activated by a change in membrane potential or activation of stretch-dependent ion channels in the plasma membrane. Phosphorylation of the 20-kDa light chain of myosin by myosin light chain kinase (MLC kinase) enables the interaction between myosin and actin. Cross-bridging of myosin with actin uses the energy released from ATP by...