Yeast lysates were analyzed for expression of E proteins by Western blotting using mAb D2-1F1-3 and 3H5. A ~50 kDa recombinant E protein band was detected in the lysates of yeast clones (Fig1.b). The lysates of yeast cells expressing DENV-1 and -2 VLPs were analyzed using a 5-50% sucrose density gradient centrifugation. The result by Western blotting elicited that DENV-VLPs was present in the faction 20%-25% (Fig1.c). After the fractions containing VLPs were harvested and mixed, the...
In order to study the effect of salinity and salicylic acid treatments on seed germination characteristics of artichoke, a laboratory study was conducted in Agriculture and Natural Resources Research Center, Hamadan. The test was implemented in factorial experiments based on a completely randomized design with three replications. The first factor was salicylic acid in 4 levels (0, 0.5, 1 and 1.5 mM) and the second factor was sodium chloride (NaCl) solution in 4 levels (0, 50, 100 and 150 mM)....
Statement of problem: The use of tooth bleaching agents has been very popular treatment in dentistry. The bleaching agents have an inherent potential to impair surface properties of existing composite resin restorations. Purpose: This study evaluated the effect of a combined bleaching regimen on the surface microhardness of a silorane-based and a sealed methacrylate-based composites. Materials and Method: Forty-five specimens of methacrylate-based composite (Ice) and 18 specimens of Silorane...
At present there is a demand for highly refined wine with special and fresh quality attributes. The use of yeasts with improved and desired properties is becoming a very important part of Enology. The influence of yeast on the phenolic content and colour of wine is considered as an important criterian for yeast selection. The experiment was conducted to determine the effects of different types of yeast on grape must from a single source. All the parameters were kept the same by varying the type...
Development of validated UV-Visible Spectroscopic, Spectrofluorimetric, HPLC and HPTLC methods for Certain Selected Drugs in Formulations and Biological Fluids. Introduction As the technology is developing, a number of new drugs are launched in the market and it becomes necessary to develop newer analytical methods. It is necessary to do if, no analytical methods are available for a drug in official books, no literature reveals methods for the determination of drugs, analytical method available...
Enzymes are protein molecules which make things happen to other molecules that would otherwise remain absolutely static. They make stable carbohydrates, fats, and proteins susceptible to digestion products as building blocks to make new cells(Komberg). A protein is classified as an enzyme if it is know to catalyze a reaction. Some enzymes are purely proteins. In other cases consist of two parts: the protein portion and a cofactor. The cofactor may be an ion of a metal or an organic molecule...
The microarray GeneChip technology was used for transcriptomics studies on ginseng and rubber including both different source of bio-material origins and comparing different growth stages through the Xspecies method. Hammond et al. (2005) described the Xspecies method as a useful method to explore genomic DNA hybridization on species that have no GeneChip designed for them, using the Affymetrix ATH1 GeneChip which was initially developed for Arabidopsis thaliana. In other words, the Xspecies...
In an ever changing world, there are more and more threats to wildlife and the need for conservation is at its greatest. Polar bears are one of the flagship species of conservation and there are many threats to their species that lead to a decline their in populations. The polar bear is the only land mammal species that uses the Arctic as its main habitat. Despite inhabiting one of least accessible areas on Earth to humans, the polar bear is been extremely vulnerable to anthropogenic impacts...
The purpose of this research report is to discuss the Exxon Valdez oil spill from several different aspects.  This incident did a tremendous amount of damage to Prince Williams Sound, but that was only the beginning. The spill affected the ocean, the animals, the people and many more. A prevention for future oil spills, response competence, and created awareness for these problems throughout the world.  In this assignment, explanations and reasons the spill occurred, what affects it had on...
Traditional medicine is based on the traditional knowledge which is held by indigenous cultures. Development of these natural medicines, used to maintain health, was largely due to trail, error, theory and belief processes (World Health Organisation (WHO), 2008). Plants have evidently been used for medicinal purposes for 60,000 years as reviewed by Cowan (1999) and Grossel-Williams et al. (2006) and as natural medicines or dietary supplements in every culture for thousands of years. The...