This chapter consists of experimental results and their explanations. We deposited composite films using pulsed DC glow discharge. Two materials such as metal and polymer are used as target whereas stainless steel 304 is used as substrate. Composite films (metallized plasma polymers) are deposited due to outstanding applications like biocompatibility, wettability, adhesion, permeability, friction and wear. However polymers frequently lack the necessary surface properties. Different techniques...
In the Cote d'Ivoire, also called the Ivory Coast, is where my step-mother was raised as a child. She remembers the horrifying stores stories of blood-eyed village man that turned to liquid. She has never experienced an Ebola outbreak, but always feared its coming. The name Ebola is one that strikes fear in the hearts of many and conjures images of people with blood coming out of all orifices of the body. Fueled by the cult classic movie Outbreak, featuring Dutsin Hoffman and Morgan freeman,...
Food safety, satisfaction and quality have always been an important concern of consumers. Several pathogens such as E. coli (Shiga toxin and verocytotoxin-producers), S. aureus, etc are recognized as important human pathogens of animal origin that are responsible for mastitis, bloody diarrhea, haemorrhagic colitis and haemolytic uraemic syndrome in humans and constitute the most important causes of food-borne disease and therefore is a major health concern and is the most economically important...
The research question is Does the amount of topspin affect the horizontal range of a ball. , the reason which I decided to research this question is because I wondered if there was any actual difference by the use of techniques which are used by professional players. The problem which I am trying to find out is the distance in which the ball hits the ground. The problem was solved by using video cameras and measuring the displacement of the ball from the machine. By solving it via computer...
Herpes Simplex Virus is a DNA virus that can propagate in various cells in multiple species. The pervasiveness of this virus in the various species is due to a number of immune-overriding mechanisms. One such mechanism is the purported degeneration of TRIM19/PML, an intracellular protein with antiviral properties that has been known to hamper viral replication, and would thus reduce the restriction of HSV infection. TRIM19/PML is a part of the tripartite motif (TRIM) family of proteins which...
study examined the effect of cholesterol esterase on the degradation of commercial poly and poly. Unstrained PEU and PCU films were incubated in CE solution or a buffer control. The study used a concentration of cholesterol esterase that was considerably higher than the estimated physiological level in order to accelerate degradation. However, characterization of treated polyurethane films with SEM, attenuated total reflectance Fourier transform infrared (ATR-FTIR) and GPC analysis revealed...
Describe how you would identify the wheat asparagine synthetase genes based on homology to genes from other species, clone the cDNAs and confirm gene function. I would firstly obtain the sequences for the Arabidopsis thaliana and rice (Oryza sativa) asparagine synthetase (AS) genes from Pubmed, and compare this to the wheat genome sequence using BLAST online. This can be seen for the wheat AS genes: TaASN1 and TaASN2 and putative homologues have been described on Homologene on the Pubmed (NCBI)...
The aim of the practical is to study the transfer of plasmid pUC18 into Escherichia. coli strain DH5α via the process of artificial transformation. Plasmid pUC18 is a 2.7kb genetically engineered plasmid, which consists of an ampicillin resistance gene (ampR) and a gene for the enzyme beta-galactosidase (lac Z). Artificial transformation is the process by which competent bacterial cells take up extracellular DNA with the help of special techniques. The growth of these E. coli colonies is...
Natamycin is a natural antimycotic, produced by fermentation of the actinomycete bacterium Streptomyces natalensis or other related species such as Streptomyces Gilvosporeus in a carbohydrate-based medium. Natamycin is also known as pimaricin and it is used as a food preservative in a variety of foods and beverages worldwide. It is a white/cream-colored powder with no taste and little odor [25]. Natamycin has the empirical formula C33H47NO13 and a molecular weight of 665,7 g/mol. It has a...
Allergic asthma is a chronic inflammatory disease that affects the larger, conducting, airways. In the last decades the incidence of allergic asthma has dramatically increased. The pathology of allergic asthma is characterized by a T helper 2 cell inflammation against an allergen, the inflammation leads to IgE production. The produced IgE antibodies will prime the mast cells, with will lead to airway hyper responsiveness. Other characteristics of allergic asthma are extensive mucus production,...