Trace The Evolutionary History Of Nicotiana Biology

This experiment is to study evolutionary history of Nicotiana by using the variation in size of intron of the QPT gene. Various techniques are use in this experiment which is DNA extraction, gel electrophoresis, PCR, nanodrop and UV spectrometry. The objective of this experiment is to determine whether genome duplication occurred in ancestral species that give rise to modern Nicotiana. Gel electrophoresis help to determine the quality of DNA extracted. UV spectrometry and nanodrop are used to determine the quantity of the nucleic acids. PCR and gel electrophoresis are used to study the evolutionary history of Nicotiana which support that whether genome duplication occurred in ancestral species that give rise to modern Nicotiana. Besides, this experiment also supports that Nicotiana tabacum which is the commercial tobacco that produce through hybridization of Nicotiana sylvestris and Nicotiana tomentosiformis.

Tobacco plants are the common name for Nicotiana species in Nicotiana genus, which consist of about 70 species. The leaf of these plants is use to make tobacco product such as cigarette. Nicotiana is a native plant in Americas, Australia, some South Pacific islands and South West Africa. Nicotiana species have very large genome and there are some research that show that that ancestral gene duplication in Nicotiana genome in the early time of its evolutionary history (Ren & Timko, 2001).

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