Iron Treatment And Pharmacological Interventions Biology

Iron overload was induced by feeding WT and HT mice with FE diet , whereas WT and HT mice in the control group were fed with a normal diet for 90 days. Then, the mice in the FE group were randomly divided into six subgroups (n=8 each), and were treated with drugs while being fed an FE diet for another 30 days as shown in Figure 2-1.

Mice in the control group were injected with 0.5% DMSO intraperitoneal (IP) daily and fed with the normal diet. Group I (FE) mice were given IP injections with 0.5% DMSO daily. Group II (FE/DFO) mice were injected with deferoxamine (DFO, 42 mg/kg) in 0.5% DMSO IP daily (Walker, et al 2009). Group III (FE/Verapamil) mice were injected with verapamil (10 mg/kg) in 0.5% DMSO twice daily (alternating subcutaneous and intraperitoneal) (Paquette, et al 1999). Group IV (FE/Nifedipine) mice were injected with nifedipine (5 mg/kg) in 0.5% DMSO IP daily (Ludwiczek, et al 2007). Group V (FE/Efonidipine) mice were injected with efonidipine (4 mg/kg) in 0.5% DMSO IP daily (Matsumura, et al 1995). Group VI (FE/Ebselen) mice were injected with ebselen (5 mg/kg) in 0.5% DMSO IP daily (Dhanarajan, et al 2006). Each group of mice were injected with drug at 6 pm each day except Fe/Verapamil group were injected twice daily at 6 am and 6 pm each day.

Figure 2-1 Timeline of iron loading and pharmacological interventions in WT and HT mice.

Blood sample was drawn from tail's vein every 30 days and collected in Na-heparin tube. Plasma were obtained by centrifugation of the blood at 3,000 rpm, 4-C for 15 minutes and kept frozen at -20-C until analysis. At the end, the treated mice were sacrificed and heart blood samples were collected. Vital organs including heart, liver, spleen and kidney were removed and weighed. Weight index were determined by using the below equation:

Weight index (%)

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